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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 40-2

40-2

ANTIBACTERIAL AND ANTIBIOFILM ACTIVITY OF p-COUMARIC ACID AGAINST MULTIDRUG-RESISTANT Acinetobacter baumannii ISOLATES

Autores:
Alisson Teixeira da Silva (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ) ; Samily Aquino de Sá Oliveira (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ) ; Edilson do Carmo Marins Júnior (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ) ; Danillo Sales Rosa (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ) ; Renata de Faria Silva Souza (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ) ; João Pedro Coelho Pereira Granja (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO ) ; Mateus Matiuzzi da Costa (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO )

Resumo:
Acinetobacter baumannii is an opportunistic bacterial pathogen frequently associated with hospital-acquired infections. Its ability to form biofilms on abiotic surfaces and human tissues contributes to its virulence and resistance to conventional antimicrobials. In this context, the search for new therapeutic agents with activity against A. baumannii biofilms is of utmost importance. p-Coumaric acid, a phenolic compound found in various plants, such as the leaves of Hymenaea martiana (jatobá), has shown antimicrobial activity in preliminary studies. However, its efficacy against biofilms has not been fully elucidated. This study aimed to investigate the antibacterial and antibiofilm activity of p-coumaric acid against multidrug-resistant A. baumannii isolates. Nine clinical multidrug-resistant A. baumannii isolates were used, obtained from the bacterial collection of the University Hospital of the Federal University of São Francisco Valley (SisGen A92B495). The antibacterial activity of p-coumaric acid was evaluated through broth microdilution assay, obtaining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The biofilm production and antibiofilm activity of p-coumaric acid were determined by the microplate adherence test and crystal violet staining, using subinhibitory concentration of ½ MIC. All assays were performed in triplicate, both technically and biologically. The p-coumaric acid exhibited bactericidal activity, with MIC and MBC ranging from 1,250 to 2,500 µg/mL. It also showed significant activity against A. baumannii biofilm formation, especially in isolates 199 and 324, with a statistically significant reduction, both transitioning from moderate to weak biofilm production. The ability of p-coumaric acid to impair biofilm formation suggests that this compound could be a promising therapeutic agent to combat resistant A. baumannii. However, further studies, including in vivo and cytotoxicity assays, are required to validate the clinical potential of p-coumaric acid as an alternative therapeutic approach to treat infections caused by multidrug-resistant A. baumannii.

Palavras-chave:
 Multidrug-resistant bacteria, Hospital-acquired Infections, Biofilm, Phenolic compound, Natural product


Agência de fomento:
Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) e Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).